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Enantiomeric separations by capillary electrophoresis in pharmaceutical analysis, Acta Universitatis Uppsaliensis 1999, Comprehensive Summaries of Uppsala Dissertations from the Faculty of Pharmacy 214. Supervisors: Prof. Douglas Westerlund, Dr. Kerstin Gröningsson, Dr. Torbjörn Arvidsson

  • Capillary Electrophoresis in pharmaceutical analysis – CE Method Validation and the use of CE in the pharmaceutical industry, Jagiellonian University Krakow, 22 Feb 2019
  • Capillary Electrophoresis in pharmaceutical analysis – CE Method Development and optimization, Jagiellonian University Krakow, 21 Feb 2019
  • Capillary Electrophoresis in pharmaceutical analysis – Pharmaceutical Analysis and CE Method Development, Jagiellonian University Krakow, 20 Feb 2019
  • Capillary Electrophoresis in pharmaceutical analysis – Different operating modes of CE, Jagiellonian University Krakow, 19 Feb 2019
  • Capillary Electrophoresis in pharmaceutical analysis – The basics of CE, Jagiellonian University Krakow, 18 Feb 2019
  • Sense and nonsense in Capillary Electrophoresis, KNCV SAC Werkgroep Scheidingsmethoden, 15 Feb 2019, Leiden
  • What is a good analytical method? Vrije Universiteit 19 Dec 2018
  • Method development and Analytical Quality by Design in separation science (CE and HPLC) applied to the analysis of pharmaceutical compounds in formulations and biological materials, Uppsala University, 11 Dec 2018
  • Regulatory aspects on Pharmaceutical analysis, Uppsala University, 10 Dec 2018
  • There is more CE for proteins! CEPharm 2018, San Francisco
  • Applications of CE to the analysis of protein therapeutics, CEPharm 2018, San Francisco
  • Why use capillary electrophoresis? Webinar Analytical Scientist, 30 Aug 2018
  • Analytical Quality by Design – an overview, Synthon 29 Aug 2018, Nijmegen
  • Good Analytical Practice, Vrije Universiteit, 4 May 2018
  • Analytical QbD Method Development for a CZE Method for Quantifying Virus Particles, webinar LC-GC, 16 Apr 2018
  • Capillary Electrophoresis in pharmaceutical analysis – CE Method Validation and the use of CE in the pharmaceutical industry, Jagiellonian University Krakow, Feb 2018
  • Capillary Electrophoresis in pharmaceutical analysis – CE Method Development and optimization, Jagiellonian University Krakow, Feb 2018
  • Capillary Electrophoresis in pharmaceutical analysis – Pharmaceutical Analysis and CE Method Development, Jagiellonian University Krakow, Feb 2018
  • Capillary Electrophoresis in pharmaceutical analysis – Different operating modes of CE, Jagiellonian University Krakow, Feb 2018
  • Capillary Electrophoresis in pharmaceutical analysis – The basics of CE, Jagiellonian University Krakow, Feb 2018
  • Buffers, Vrije Universiteit 19 Jan 2018
  • AQbD – speeding up or slowing down? The Netherlands area biotech discussion group, 7 Dec 2017
  • Novel CZE method for the quantification of intact adenovirus particles - AQbD method development and implementation, HPLC2017 Jeju
  • Robust CE methods - excerpts from good practice in CE method development and validation, HPLC2017 Jeju
  • Pharmaceutical analysis, Vrije Universiteit 2017
  • Method development - some general remarks, Polymun 2017
  • AQbD and CE: speeding up or slowing down? CEPharm 2017
  • CE troubleshooting, CEPharm 2017
  • Applications of capillary electrophoresis to the analysis of protein therapeutics, CEPharm 2017
  • Capillary Electrophoresis, Uppsala University 2017
  • Analytical method development and validation, Uppsala University 2017
  • Robust CE: method development and good working practices, Euroanalysis 2017
  • Basic capillary electrophoresis, Euroanalysis 2017
  • Robust CE - is it possible? Excerpts from good practice in CE method development and validation, Sciex User Meeting 2017
  • Analytical Quality by Desing and capillary electrophoresis: do they match? LUMC 2017
  • Two sides of science, MSB2017
  • Robust CE: method development and good working practices, MSB2017
  • Homeopatische geneesmiddelen, 8 - 10 meeting, 2016
  • CE troubleshooting, CEPharm 2016
  • Applications of Capillary Electrophoresis to the analysis of protein therapeutics, CEPharm 2016
  • Reliable methods in CE: Method development and good working practices, FACE 2016
  • Joining the scientific and industrial worlds together - Implementation of Capillary Electrophoresis, FACE 2016
  • Quality by Design for Analytical Method Development, MSB2016
  • CASA Farmacia, CASA Campagna 2016
  • Capillary Electrophoresis supporting viral vaccine development, HTC-14, 2016
  • Reliable Methods in CE: Fundamentals and Good Working Practices, ATEurope 2015
  • Geneesmiddelontwikkeling, Het Baarnsch Lyceum, 2015
  • CE method transfer, from R&D to QC, CE seminar - See what you've been missing, Analis, Namur, Oct 2014
  • Pharmaceutical analysis – a practical drug development point of view, University of Salzburg, 2014
  • Quality by Design – The Use of Experimental Designs in Capillary Electrophoresis Method Development and Validation, QbD eSeminar, 2014
  • Polarization of the use of the CE techniques in pharma industry?, Chromatographic Society Spring Symposium, Macclesfield 2014
  • Quality by Design and CE – Points of Attention, CEPharm 2013, Arlington 2013
  • Validation in CE – Points of Attention, Beckman seminar Basel, 2013
  • Quality by Design and Good CE Working Practice, PBA 2013, Bologna 2013
  • Revival of the CE techniques in the Pharmaceutical Industry, KNCV WGS, 2013
  • Troubleshooting in Capillary Electrophoresis, CE User Meeting 2012, 2013, 2014
  • CE method development, validation and good working practices — The do’s and don’ts to make a CE method robust, sensitive and precise, MSB2012 Post Conference Short-Course, 2012
  • Reliable Methods in CE - Method Validation and Good Working Practices, BeckmanCoulter short course, 2012, 2013
  • Quality by Design through good working practices in CE, CE Forum 2012, Aalen, 2012
  • Pharmaceutical Analysis and Capillary Electrophoresis, Uppsala University, 2012
  • CE in the Pharmaceutical and Biotech Industry – Myths, facts, use, issues and needs, ITP2011, Tbilisi, Georgia, 2011; MSB2012, Geneva, 2012; Separation Science Asia 2012, Kuala Lumpur, 2012
  • Enantiomeric separations in pharmaceutical analysis: CD-MEKC for acidic chiral drug compounds, Dutch Royal Society of Chemistry (KNCV) CE User Meeting, 2011
  • Chiral Capillary Electrophoresis, Beckman UK workshop, 2011
  • Additional examples of anion and cation analysis with CE, Beckman UK workshop, 2011
  • Validation and capillary electrophoresis methods, Beckman UK workshop, 2011
  • Capillary Electrophoresis - The basics, Beckman UK workshop, 2011
  • Capillary Electrophoresis in the Pharmaceutical and Biotech Industry, Masterclass Avans Hogeschool, 2011
  • Method Development, Optimization and Good Working Practice in Capillary Electrophoresis, short course CEPharm 2010 – current
  • Dissolution, a versatile tool in Drug Development, Workshop Weesp, 2010
  • Scheikunde, lecture and workshop at ground school with students aged 10-11 years old, 2009, 2012
  • Method development & QbD, CEPharm 2009 workshop, 2009
  • Recent applications of CE-techniques within Solvay Pharmaceuticals, CEPharm 2009
  • Improving CE methods for small molecules with "Good CE Practice" , MSB2008 short course, 2008
  • Capillary Electrophoresis: theory, modes, practice and method development, Agilent in-company short course, 2008
  • “Good CE Practice” – Ways to improve your CE method performance, CEPharm 2007
  • Developing and validating capillary electrophoresis for pharmaceutical analysis, Analytical Method Validation, 26 – 27 Oct 2006, Prague, 2006
  • ”Good CE Practice”, short course MSB 2006
  • Enantiomeric purity determination of Adrenaline at low concentrations in Local Anaesthetic solutions, HPLC 2005
  • The use of CE in pharmaceutical analysis at AstraZeneca, Analysdagarna 2003
  • The implementation of CE techniques in pharmaceutical analysis at AstraZeneca, CEPharm 2002, HPCE 2002
  • CE in pharmaceutical analysis, Stockholm Univsersitet, seminari i doktorandkurs, 2000
  • Validation in (chiral) capillary electrophoresis, Analysdagarna 1999
  • Extension of the model for chiral CE by complex formation of one enantiomer molecule with more than one chiral selector molecules, Fifth International Symposium on Capillary Electrophoresis including 2nd International Meeting on Capillary Electrochromatography, York, 1998
  • Capillary electrophoresis at Astra Pain Control AB - Method development and validation of chiral capillary electrophoresis, HP Capillary Separations seminar, 1996
  • Chiral separation of local anaesthetics with capillary electrophoresis - Evaluation of the inclusion complex, Analysdagarna 1996
  • Enantiomeric separations with capillary electrophoresis, Validation in capillary electrophoresis course, University of York, 1996
  • CE-FPD Capillary electrophoresis with flame photometric detection, Dutch Royal Society of Chemistry (KNCV) CE User Meeting, 1994

 

  • Ewoud van Tricht, Lars Geurink, Francisca Galindo Garre, Martijn Schenning, Harold Backus, marta Germano, Govert Somsen, Cari Sänger – van de Griend
    Implementation of at-line capillary zone electrophoresis for fast and reliable determination of adenovirus concentrations in vaccine manufacturing
    Electrophoresis, 2019, accepted for publication
  • Prasanta Paul, Cari Sänger – van de Griend, Erwin Adams, Ann Van Schepdael
    Recent advances in the capillary electrophoresis analysis of antibiotics with capacitively coupled contactless conductivity detection
    J Pharm Biomed Anal 158 (2018) 405 – 415
  • Paul Prasanta, Cari Sänger-van de Griend Cari, Erwin Adams, Ann Van Schepdael
    Development and validation of a CE method for the determination of tetracyclines with capacitively coupled contactless conductivity detection
    Journal of Pharmaceutical and Biomedical Analysis 158 (2018) 405 – 415
  • Ewoud van Tricht, Pascal de Raadt, Annemiek Verwilligen, Jonathan Knibbe, Martijn Schenning, Harold Backus, Marta Germano, Govert W. Somsen, Cari E. Sänger – van de Griend
    Fast, selective and quantitative protein profiling of adenovirus-vector based vaccines by UPLC
    J Chromatogr. A 1581-1582 (2018) 25 – 32
  • Prasanta Paul, Cari Sänger-van de Griend, Erwin Adams, Ann Van Schepdael
    A simple, low-cost and robust capillary zone electrophoresis method with capacitively coupled contactless conductivity detection for the routine determination of four selected penicillins in money-constrained laboratories
    Electrophoresis 39 (2018) 2521 – 2529
  • Han Yao, Jana Vandenbossche, Cari E.  Sänger - van de Griend, Yorick  Janssens, Cristina  Soto Fernández, Xiaolong  Xu, Evelien  Wynendaele, Govert  W Somsen, Rob  Haselberg, Bart de Spiegeleer
    Development of a capillary zone electrophoresis method to quantify E. coli L-asparaginase and its acidic variants
    Talanta 182 (2018) 83 - 91
  • Prasanta Paul, Tom Duchateau, Cari Sänger-van de Griend, Erwin Adams, Ann Van Schepdael
    CE-Capacitively coupled contactless conductivity detection method development and validation for the determination of azithromycin, clarithromycin and clindamycin
    J. Sep. Sci. 40 (2017) 3535 – 3544
  • Ewoud van Tricht, Lars Geurink, Marta Germano, Govert W. Somsen, Cari E. Sänger – van de Griend
    One single, fast and robust capillary electrophoresis method for the direct quantification of intact adenovirus particles in upstream and downstream processing samples
    Talanta 166 (2017) 8 - 14
  • Prasanta Paul, Christophe Van Laeken, Cari Sänger-van de Griend, Erwin Adams, Ann Van Schepdael
    CE-C4D method development and validation for the assay of ciprofloxacin
    J Pharm Biomed Anal 129 (2016) 1 – 8
  • Ewoud van Tricht, Lars Geurink, Bojana Pajic, Johan Nijenhuis, Harold Backus, Marta Germano, Govert W. Somsen, Cari E. Sänger-van de Griend
    New capillary gel electrophoresis method for fast and accurate identification and quantification of multiple viral proteins in influenza vaccines
    Talanta 144 (2015) 1030 – 1035
  • Cari E. Sänger – van de Griend, Anders G. Ek, Monica Widahl – Näsman, E.K. Margareta Andersson
    Method development for the enantiomeric purity determination of low concentrations of adrenaline in local anaesthetic solutions by capillary electrophoresis
    J. Pharm. Biomed. Anal. 41 (2006) 77 – 83
  • Jens Ohnesorge, Cari Sänger – van de Griend, Hermann Wätzig
    Quantification in Capillary Electrophoresis-Mass Spectrometry: long- and short-term variance components and their compensation using Internal Standards
    Electrophoresis 26 (2005) 2360 – 2375
  • Falko Süß, Valérie Harang, Cari E. Sänger – van de Griend and Gerhard K. E. Scriba
    Development of a robust chiral capillary electrophoresis assay for calcium levofolinate including related substances using statistical experimental design
    Electrophoresis 25 (2004) 766 – 777
  • Valérie Harang, Jessica Eriksson, Cari E. Sänger – van de Griend, Sven P. Jacobsson, Douglas Westerlund
    Microemulsion electrokinetic chromatography of drugs varying in charge and hydrophobicity – Part I: Impact of parameters on separation performance evaluated by multiple linear regression models
    Electrophoresis 25 (2004) 80 – 93
  • Falko Süß, Cari E. Sänger – van de Griend, Gerhard K.E. Scriba
    Migration order of peptide enantiomers in the presence of single isomer and randomly sulfated cyclodextrins as a function of pH
    Electrophoresis 24 (2003) 1069 – 1076
  • Leonhard Baur, Cari Sänger – van de Griend, Hermann Wätzig
    Electroosmotic flow variations caused by the volatility of buffer components: diagnosis and therapy
    J. Chromatogr. A 979 (2002) 97 – 103
  • Olle Stålberg, Karin Sander, Cari Sänger – van de Griend
    The determination of bromide in a local anaesthetic hydrochloride by capillary electrophoresis using direct UV detection
    J. Chromatogr. A 977 (2002) 265 – 275
  • F. Süß, W. Poppitz, C.E. Sänger – van de Griend and G.K.E. Scriba
    Influence of the amino acid sequence and nature of the cyclodextrin on the separation of small peptide enantiomers by capillary electrophoresis using randomly substituted and single isomer sulfated and sulfonated cyclodextrins
    Electrophoresis 22 (2001) 2416 – 2423
  • C.E. Sänger – van de Griend
    Enantiomeric separation of alanyl and leucyl dipeptides by capillary electrophoresis with cyclodextrins as chiral selectors
    Electrophoresis 21 (2000) 2397 – 2404
  • C.E. Sänger – van de Griend
    Enantiomeric separation of glycyl dipeptides by capillary electrophoresis with cyclodextrins as chiral selectors
    Electrophoresis 20 (1999) 3417 – 3423
  • C.E. Sänger – van de Griend, K. Gröningsson and T. Arvidsson
    Enantiomeric separation of a tetrapeptide with cyclodextrin – Extension of the model for chiral capillary electrophoresis by complex formation of one enantiomer molecule with more than one chiral selector molecules
    J. Chromatogr. A 782 (1997) 271 – 279
  • C.E. Sänger – van de Griend, H. Wahlström, K. Gröningsson and M. Widahl – Näsman
    A chiral capillary electrophoresis method for ropivacaine hydrochloride in pharmaceutical formulations: validation and comparison with chiral liquid chromatography
    J. Pharm. Biomed. Anal. 15 (1997) 1051 – 1061
  • C.E. Sänger – van de Griend, K. Gröningsson and D. Westerlund
    Chiral separation of local anaesthetics with capillary electrophoresis – Evaluation of the inclusion complex of the enantiomers with heptakis(2,6-di-O-methyl)-β-cyclodextrin
    Chromatographia 42 (1996) 263 – 268
  • C.E. Sänger – van de Griend and K. Gröningsson
    Validation of a capillary electrophoresis method for the enantiomeric purity testing of Ropivacaine, a new local anaesthetic compound
    J. Pharm. Biomed. Anal. 14 (1996) 295 – 304
  • C.E. Sänger – van de Griend, Ch.E. Kientz and U.A.Th. Brinkman
    Capillary electrophoresis coupled on-line with flame photometric detection
    J. Chromatogr. A 673 (1994) 299 – 302

2010 - present

  • Capillary Electrophoresis – a part of iConsensus, an integrated control and sensing platform for biopharmaceutical cultivation processes
  • Method puberty; adenovirus quantification CZE method implementation challenges
  • The development of a bromide quantification method for viral vaccine development
  • The implementation of a new method development process
  • A multipurpose adenovirus CZE method
  • Quality Methods by Design
  • Novel CZE Method for the Quantification of Intact Adenovirus Particles – AQbD Implementation and Application
  • Influenza virus proteins CGE method development; the effects of the injection mode and gel buffer dilution
  • Toward robust analysis of human growth hormone by capillary electrophoresis-mass spectrometry - essential considerations
  • Implementation of capillary electrophoresis for viral vacine development support in a GMP environment
  • Novel CZE method for the quantification if intact virus particles in complec matrices - Quality by Design method development
  • Enriching the analytical toolbox for viral vaccines with capillary electrophoresis
  • Improved method for quantification of virus particles in complex matrices using capillary electrophoresis
  • Novel method for the quantification of adenovirus particles in complex matrices using capillary zone electrophoresis
  • How to access the performance of capillary electrophoresis instruments
  • Capillary Electrophoresis: an Attractive Technique for Chiral Separations
  • Capillary Gel Electrophoresis for Analysis of Polio Virus - Development and Qualification in 4 Days
  • In-Capillary sample concentration in CE – “This is my analyte, how do I stack?”
  • Capillary Gel Electrophoresis for Analysis of Influenza Virus – The effects of injection mode and gel buffer dilution
  • QbD and CE: some practical points for attention
  • Quality by Design and Good Working Practices for CE
  • Capillary Gel Electrophoresis for Analysis of Influenza Virus – Development of an orthogonal method for influenza identity and HA quantification
  • Viral vaccine characterization with capillary electrophoresis
  • 50 Ways to improve your CE method
  • Comparison of chiral NACE with CD-MEKC using a chiral acid as model compound

2000 - 2010

  • Microemulsion electrokinetic chromatography: Investigation of the applicability on Solvay drug products
  • Quality by Design: Multi-factorial optimization of a CD-MEKC method for the chiral separation of an acidic drug substance and its formulations
  • Development and validation of the chiral separation of an acidic pharmaceutical compound and its enantiomer with CD-MEKC
  • Development of a dissolution method for in vitro-in vivo correlation of BCS class I and II drugs
  • Towards precise and validated bioanalysis – CE‑MS, protein adsorption, HPLC assays
  • Enantiomeric purity determination of Adrenaline at low concentrations in Local Anaesthetic solutions
  • Long term precision in capillary electrophoresis electrospray mass-spectrometry
  • Long term system testing of precision using a capillary electrophoresis electrospray mass-spectrometry standard method
  • Microemulsion electrokinetic chromatography of drugs varying in charge and hydrophobicity. Impact of parameters on separation performance evaluated by multiple linear levofolinate including related substances using statistical experimental design regression models
  • Development of a robust chiral capillary electrophoresis method for calcium
  • Separation of dipeptide and tripeptide enantiomers in capillary electrophoresis using cyclodextrins
  • EOF variations caused by the volatility of buffer components: diagnosis and therapy

1993 - 1999

  • Enantiomeric separation of underivatised dipeptides with capillary electrophoresis II. Alanyl and leucyl dipeptides
  • Enantiomeric separation of underivatised dipeptides with capillary electrophoresis I. Glycyl dipeptides
  • Extension of the model for chiral separations in capillary electrophoresis
  • Determination of bromide in a local anaesthetic drug by capillary electrophoresis using direct UV-detection and acetonitril for improvement of the selectivity of the method
  • Comparison of  Capillary Electrophoresis with Liquid Chromatography for the Enantiomeric Purity Determination of Ropivacaine Hydrochloride - Extended method comparison: introduction of the CE technique for routine analysis
  • Extension of the model for chiral CE by complex formation of one enantiomer molecule with more than one chiral selector molecule
  • Enantiomeric separation of a tetrapeptide - Extension of the theoretical model for chiral separations in capillary electrophoresis
  • Robust methods in Capillary Electrophoresis - Robustness of a method for determination of the enantiomeric purity of ropivacaine hydrochloride in pharmaceutical formulations
  • Comparison of CE with LC in the enantiomeric purity determination of ropivacaine
  • Chiral separation of local anaesthetics with capillary electrophoresis - Evaluation of the inclusion complex
  • Validation of the CE method for the enantiomeric purity testing of ropivacaine
  • The enantiomeric separation of local anaesthetics with capillary electrophoresis
  • Capillary liquid separation techniques coupled to flame-based gas chromatographic detectors
  • Improved interface for the coupling of capillary liquid separation techniques to flame-based gas chromatographic detectors